Chlorogenic acid prevented a stepwise conversion of plasmid pUC18 DNA, from I-->form II-->form III, induced by 3 mM monochloramine with a half inhibition of 67.4 microM. Chlorogenic acid reacted with monochloramine in a time-dependent manner, and the reaction rate increased with decreasing pH. These results suggest that chlorogenic acid prevents genotoxicity of monochloramine in gastric mucosa.
The natural polyphenols found in green coffee beans are a group of hydroxycinnamic acids, the primary ones being chlorogenic, caffeic and ferulic acids. This group of polyphenols has many significant health benefits. Aside from their potent antioxidant activity, the most recently studied benefits pertain to their potential role in carbohydrate metabolism. Chlorogenic acid specifically is shown to decrease the uptake of glucose in the body and assist in regulating blood glucose levels.
The results from a clinical study performed in 12 healthy volunteers with different coffee products containing glucose show that instant coffee enriched with chlorogenic acid induced a reduction in the absorption of glucose of 6.9% compared with the control. No such effects were seen with normal or decaffeinated instant coffee. In a second, comparative, randomized, double-blind, 12-week study we investigated the effect on the body mass of 30 overweight people, compared with normal instant coffee. The average losses in mass in the chlorogenic acid enriched and normal instant coffee groups were 5.4 and 1.7 kg, respectively. We conclude that chlorogenic acid enriched instant coffee appears to have a significant effect on the absorption and utilization of glucose from the diet. This effect, if the coffee is used for an extended time, may result in reduced body mass and body fat when compared with the use of normal instant coffee.
In a recent issue of Pharmaceutical Biology, researchers noted that combinations of four compounds from St. John''s wort (amentoflavone, chlorogenic acid, pseudohypericin and quercetin) were more effective at reducing inflammation in mouse macrophage assays than when each was used alone.
Chlorogenic acids are cholegogues; their regular ingestion helps the flow of bile and thus reduces the adverse effects of bile stagnation. Chlorogenic acids (see basic structure, below) are found in all higher plants and are understood to have a role in the plant''''s response to stress, particularly to damage such as breaking of leaves and flowers or nicking the skin of fruits (1). The quantities present in most plants are miniscule, and thus they do not play an important part in human diet or herbal medicines.
ObjectiveTo establish a spectrophotometric method for determination of chlorogenic acid in Eucommia Ulmoides leaves.Methods The complex chromogenic reaction of chlorogenic acid and Al3+ was applied for the determination with visible spectrophotometer at 530 nm after chlorogenic acid was extracted by thin-layer chromatography. ResultsThe working curve for the determination of chlorogenic acid solution was linear in the range of 1.7×10-4～1.0×10-2g•L-1. The corresponding equation of linear regression was Y=0.003 92 X－0.0067，r=0.9995. The recovery rates of the leaves were 98% and 101% in autumn and summer, respectively. ConclusionThis method is simple, practical and accurate. It meets the requirement for the daily determination in the lab and industry.